Literature summary extracted from

Mu, T.; Liang, W.; Ju, Y.; Wang, Z.; Wang, Z.; Roycik, M.D.; Sang, Q.X.; Yu, D.; Xiang, H.; Fang, X.
Efficient soluble expression of secreted matrix metalloproteinase 26 in Brevibacillus choshinensis (2013), Protein Expr. Purif., 91, 125-133.

Application

EC Number	Application	Comment	Organism
3.4.24.B7	synthesis	use of Brevibacillus choshinensis as the host system for the soluble and active expression of MMP26. The enzyme is secreted in soluble form in the supernatant of cell culture medium. The yields of purified proform of MMP26 and catalytic form of MMP-26 are 12 and 18 mg/L, respectively, with high purity and homogeneity. Both pro- and catalytic form show gelatin zymography activity and the purified catalytic form has high enzymatic activity against DQ-gelatin substrate. Expression using several widely used expression vectors in Escheriochia coli cells results in insoluble expressions or soluble expressions with little catalytic activity	Homo sapiens

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.24.B7	expression in Brevibacillus choshinensis	Homo sapiens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.24.B7	19000	-	x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform	Homo sapiens
3.4.24.B7	28000	-	x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.24.B7	Homo sapiens	Q9NRE1	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.24.B7	recombinant protein	Homo sapiens

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.4.24.B7	133000000	-	fluorescent units/mg, pH 7.5, 22°C	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.24.B7	DQ-gelatin + H2O	fluorescein conjugated substrate from pig skin	Homo sapiens	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.24.B7	?	x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform	Homo sapiens

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Release 2023.1 (January 2023)